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Fig. 6 | Experimental Hematology & Oncology

Fig. 6

From: LINP1 represses unfolded protein response by directly inhibiting eIF2α phosphorylation to promote cutaneous squamous cell carcinoma

Fig. 6

The UPR-mediated apoptosis modulated by LINP1 is dependent on DDIT3. A The binding site of ATF4 on the promoter region of DDIT3 was predicted by rVista (https://rvista.dcode.org/) and UCSC genome browser (https://genome.ucsc.edu/). B The binding enrichment of ATF4 at the binding site on the promoter region of DDIT3 was detected by ChIP-qPCR after depletion of LINP1. C LINP1, DDIT3 and DR5 RNA expression were detected by qPCR after LINP1 knockdown and/or DDIT3 knockdown. DG Cell proliferation, colony formation assay, migration, invasiveness assays and Annexin V-APC/7-AAD double staining measurement were performed after depletion of LINP1 or/and DDIT3. H The protein levels of DDIT3, DR5 and cleaved forms of Caspase-8, Caspase-7 and Caspase-3 were detected. Each experiment was performed in at least triplicate and results are presented as mean ± s.d. One-way ANOVA and Dunnett’s multiple comparison test were used to analyze the data. (*P < 0.05, **P < 0.01, ***P < 0.001). Scale bars, 500 mm E, 100 μm F

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