Fig. 1

LINP1 is upregulated in cSCC cells and tumors and acts as oncogene. A Clustering analysis of top 10 upregulated and top 10 downregulated LncRNAs from published transcriptomic sequencing dataset (GSE139505) [23] was sorted according to the deviation values and shown in a heatmap. Color bars on the left represent ranges of z-value. LINP1 was indicated by a blue arrow. Violin plot was used to compare the normalized expression levels of LINP1 from nine cSCC tissue samples and seven unmatched normal skin samples. B The expression levels of LINP1 were detected by qPCR in HaCaT keratinocytes, primary keratinocytes and cSCC cell lines (HSC-1 and A431). C The expression levels of LINP1 were compared between normal skin tissues and cSCC tumors. The qPCR data represents the average of three independent experiments ± s.d. D ISH detection of LINP1 on paraffin sections of cSCC tumors and normal skin specimens. Representative images with various levels of LINP1 expression (weak staining from normal tissues, stronger straining from tumor tissues) were shown. Scale bar: 50 µm. E Association of LINP1 staining scores with tumor grade (Normal skin tissues, I, II, III & IV). *P < 0.05, **P < 0.01, ***P < 0.001