Skip to main content
Fig. 1 | Experimental Hematology & Oncology

Fig. 1

From: Age-related accumulation of B-1 cell progenitors in mice reflects changes in miR15a/16-1 expression and radioresistance capacity

Fig. 1

B-1 cell progenitor accumulates in middle-aged C57Bl/6 mice and presents downregulation in miR-15a/16 expression, but no difference in Bcl-2 expression. A Gates strategies used to evaluate the percentage of B-1p in bone marrow of young (above) or middle-aged (below) mice. First, the single cells were selected (SSC-A x SSC-H) and the population was defined (SSC-A x FSC-A). After that, Lin-CD93+ cells were selected followed up to CD45R-/lowCD19+ selection of B-1p phenotype. B Frequency of total B-1 progenitor cells (Lin-CD45R-/lowCD19+CD93+) present in bone marrow of young (0.071% ± 0.0249) and middle-aged mice (0.1375% ± 0.0317). Student’s t test: t(5)=3.512, p=0.0391. C Frequency of parent of B-1p cells (Lin-CD45R-/lowCD19+CD93+) present in bone marrow of young (0.6121% ± 0.4943) and middle-aged (2.5667% ±0.8958) mice. Student’s t test t(5)=3.309, p=0.0297. D Absolute number of B-1 progenitor cells (Lin-CD45R-/lowCD19+CD93+) present in bone marrow of young (88 ± 4) and middle-aged (125 ± 21.9) mice. Student’s t test t(5)=2.875, p=0.0453. Figure. 1A is representative of 3 animals per group evaluated separately. The experiment was repeated with a pool of cells from 10 animals per group. Figures 1B-D represent statistics of three independent experiments combined. E These samples were normalized with B-1p samples from young mice and the endogenous gene was u6 snRNA to miRNA and ß-2 microglobulin to apoptotic genes. 2-ΔΔCt method was used. This experiment was performed in triplicate with 4 different biological samples and the average of 4 experiments is shown. Student’s t test t(8)=2.77; p=0.033; cohend’=1.96 (high effect size). F Levels of Bcl-2 phosphorylation in young or middle-aged B-1p cells. Lanes 1 and 2 represent young B-1p and middle-aged B-1p, respectively. The graph shows the pBcl-2 and GAPDH relation. This experiment was performed with 2 biological samples (10 animals per group resulting in a pool of cells)

Back to article page