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Fig. 9 | Experimental Hematology & Oncology

Fig. 9

From: Synergistic effect of HDAC inhibitor Chidamide with Cladribine on cell cycle arrest and apoptosis by targeting HDAC2/c-Myc/RCC1 axis in acute myeloid leukemia

Fig. 9

RCC1 as the target of c-Myc in AML. (A) The expression of c-MYC and RCC1 in vector control versus shMYC-U937 cells was detected by RT-qPCR. (B) The expression of RCC1 in U937 cells and THP-1 cells treated with Chidamide (CHI) alone, Cladribine (CLA) alone, and two drugs combination for 48 h was detected by RT-qPCR. (C) Western blot of RCC1 in U937 cells and THP-1 cells that were treated by CHI alone, CLA alone, and two drugs combination for 48 h. (D) The predicted binding sequence in the promoter region of RCC1 and the motif plot of the E-box element. (E) The relative amount of precipitated RCC1 promoter DNA of the treated cells was purified by c-Myc antibody and was detected by RT-qPCR. *p < 0.05, **p < 0.01, ***p < 0.001

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Experimental Hematology & Oncology

ISSN: 2162-3619

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