Skip to main content
Fig. 2 | Experimental Hematology & Oncology

Fig. 2

From: Single-cell transcriptome analysis reveals stem cell-like subsets in the progression of Waldenström’s macroglobulinemia

Fig. 2

Stem cell-like subset in patients with WM and its mutational landscape. A Pseudo-time analysis of B cells, B cells with aberrant T cell markers, plasma cells inferred by Monocle 2. Expression of classical markers shows clusters identification: CD3 + CD20 + cells and preB cells (IGLL1 +) are located in the “root” (pseudo-time 0) and B cells (MS4A1 +) and plasma cells (SDC1 +) are located in the branch. B Flow cytometric analysis showing the population of B cells with aberrant T cell markers in two WM patients. CD3 + CD19 + cells were labeled with red color. And expression of CD4 and CD8 in CD3 + CD19 + cells were analyzed. C Mutant genes identified by target region sequencing in CD3 + CD19 + cells and CD3-CD19 + cells of 6 patients with WM. Mutation types and mutation rate of each gene were displayed in the right side. D Heatmap showing VAF distribution of nine mutated genes. E Dynamics of VAF across CD3 + CD19 + cells and CD3-CD19 + cells

Back to article page

Experimental Hematology & Oncology

ISSN: 2162-3619

Contact us