Fig. 8

Genome-wide occupancy profiles of HIF-1α and circPRDM4. A Heatmap showing the binding sites of HIF-1α by ChIP-seq assay. B ChIP-seq analysis of HIF-1α genomic binding at target sites. A 10-kb interval centered on each HIF-1α peak is shown. C Heatmap showing the binding sites of circPRDM4 by ChIRP-seq assay. The signals near the peak center of circPRDM4-bound regions are plotted. D Global representation of circPRDM4 genomic binding over a 10-kb window centered on each circPRDM4 ChIRP-seq peak. E Venn graph showing the intersecting genes by ChIRP-seq assay for circPRDM4 and ChIP assay for HIF-1α. F ChIP-seq graph of CD274 promoter bound by HIF-1α. G Representative ChIRP-seq of circPRDM4 binding of CD274. H Schematic diagram showing that circPRDM4 enhances HCC immune escape through HIF-1α regulation of PD-L1