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Fig. 3 | Experimental Hematology & Oncology

Fig. 3

From: Hypoxia-associated circPRDM4 promotes immune escape via HIF-1α regulation of PD-L1 in hepatocellular carcinoma

Fig. 3

circPRDM4 knockdown suppresses PD-L1 expression and promotes the CD8+ T cell-mediated anti-tumor immune response under hypoxic conditions. A The knockdown efficiency of circPRDM4 in MHCC97H cells. B The knockdown efficiency of circPRDM4 in HCCLM3 cells. C Expression levels of PRDM4 mRNA in circPRDM4-silenced MHCC97H and HCCLM3 cells. D Expression levels of CD274 mRNA in circPRDM4-silenced HCC cells under hypoxia. E Expression levels of PD-L1 protein in circPRDM4-silenced HCC cells under hypoxia. F Cell surface PD-L1 expression levels in circPRDM4-silenced HCC cells under hypoxia. G T cell-mediated cancer cell-killing assay results of circPRDM4-silenced HCC cells. H LDH release assay results showing the cytotoxicity in circPRDM4-silenced HCC cells under hypoxia. I ELISA assays showing secreted TNF-α and IFN-γ from T cells cocultured with circPRDM4-silenced MHCC97H and HCCLM3 cells under hypoxia. Data are shown as mean ± SEM. **, P < 0.01; ***, P < 0.001; ns, no significance

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