Fig. 6

The mechanism of GPR109A on reducing NETs formation sensitivity. a Neutrophils were isolated from WT mice and GPR109A^−/− mice, and then the ROS level of neutrophils in the two groups was detected. b–d The NETs formation after inhibition of ROS. e The bactericidal ability of NETs. The protein expression in neutrophils was detected by Western blotting. f Cit-H3 level in non stimulated mice neutrophils. g–i Cit-H3 level in neutrophils of WT mice and GPR109A^−/− mice stimulated by S. aureus, E. coli and LPS. Values are presented as means ± SEM (n = 3) (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001)