Fig. 3

Effect of GPR109A on NETs formation. a–d We stimulated neutrophils with different concentrations of E. coli, S. aureus and LPS for 30 min. The results showed that GPR109A had significant difference in the formation of NETs when MOI = 10. e–f We stimulated neutrophils with E. coli with MOI = 10. Effect of GPR109A on NETs formation at different time points. Values are presented as means ± SEM (n = 6) (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001)