Fig. 2

Isolation and identification of neutrophils. We collected neutrophils from mouse abdominal cavity, and then identified them by laser confocal microscopy and flow cytometry. The antibodies used in flow cytometry were Ly-6G and CD11b. a Identification results of neutrophils; Confocal pictures of neutrophils. b–e The gene levels of PAD4 in neutrophils in WT and GPR109A^−/− mice. Values are presented as means ± SEM (n = 3) (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)