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Fig. 4 | Experimental Hematology & Oncology

Fig. 4

From: Depletion of polyfunctional CD26highCD8+ T cells repertoire in chronic lymphocytic leukemia

Fig. 4

Cytokine production and proliferation ability of CD26neg/low/high CD8+ T cells in CLL. A Representative flow plots, (B) the frequency of TNF-\(\alpha\), (C) IFN-\(\gamma ,\) and (D) TNF-\(\alpha\)+IFN-\(\gamma\)+ cells among CD26neg/low/high CCR7−CD8+ T cells. E Representative plots, (F) cumulative data showing the frequency of TNF-\(\alpha\)+, (G) IFN-\(\gamma\)+, and (H) TNF-\(\alpha\)+IFN-\(\gamma\)+ among CD26neg/low/high CCR7−CD8+ T cells in unstimulated or stimulated with anti-CD3/CD28 (3 g\(\upmu\)/ml, 1 g\(\upmu\)/ml) and a cocktail of IL-18 + IL-12 + IL-15 (100 ng/ml of each). (I) Representative flow plots and, (J) cumulative data of the frequency of IL-2 expressing cells among CD26neg/low/high CD8+ T cells in unstimulated (black color) versus 5 h after in-vitro stimulation with PMA/ionomycin cocktail (Biolegend, 2 ng/ml) in the presence of Brefeldin A (1 g\(\mu\)/ml). (K) Representative flow plots and, (L) cumulative data of the frequency of CFSElow (proliferated) cells among CD26neg/low/high CCR7−CD8+ T cells, unstimulated (black color) versus 72 h stimulation. (M) Representative flow plots, and (N) cumulative data showing the MFI for ROR \(\gamma \delta\) in CD26neg, CD26low, and CD26high CD8+ T cells in CLL. Error bars represent the median with an interquartile range. Each dot represents an individual human sample

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