Fig. 6

PAQR4 promoted the development of HCC by activating AKT. A Silver staining indicated PAQR4 may interact with AKT. B Co-immunoprecipitation (Co-IP) assay identified the interaction between PAQR4 and AKT. C The colocalization of PAQR4 and AKT was observed by immunofluorescence in HLF and HepG2 cells. D Western blot analysis showed activation of AKT pathway with or without IGF-1 stimulation when PAQR4 was overexpressed. (E, F and Additional file 4A) CCK8 and EdU assays showed knockdown of AKT reduce proliferation caused by overexpression of PAQR4. (G, H and Additional file 4B, C) Transwell and wound healing assays showed that knockdown of AKT reduce increased migration and invasion capability caused by overexpression of PAQR4. GAPDH was used as internal controls in Western blotting analysis. *P < 0.05, **P < 0.01, ***P < 0.001