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Fig. 2 | Experimental Hematology & Oncology

Fig. 2

From: ALKBH5 prevents hepatocellular carcinoma progression by post-transcriptional inhibition of PAQR4 in an m6A dependent manner

Fig. 2

ALKBH5 suppressed m6A modification of PAQR4 mRNA. A The heat map exhibited the changed genes of transcriptome sequencing after ALKBH5 overexpression. B, C RT–qPCR analysis showed the six altered target genes with ALKBH5 knockdown or ALKBH5 overexpression. D The result of m6A abundances in mRNA transcripts of ALKBH5 target genes was shown. E Western blot analysis showed the protein level of PAQR4 with overexpression of ALKBH5 and knockdown of ALKBH5 in HCC cells. F–H RT–qPCR analysis and Western blot verified both mRNA and protein expression of PAQR4 was decreased when co-transfected with ALKBH5-WT but not with ALKBH5-H204A. GAPDH was used as internal controls in Western blotting and RT–qPCR analysis. *P < 0.05, **P < 0.01, ***P < 0.001

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