Fig. 1

ALKBH5 is decreased in HCC and suppress HCC proliferation and invasion in vitro. A Protein level of ALKBH5 was detected by Western bolt assay in paired HCC samples (n = 60). CCK8 and EdU assays confirmed that overexpression of ALKBH5 inhibit proliferation of 97H and HLF (B, D and Additional file 1A), whereas knockdown of ALKBH5 promoted cell proliferation in LM3 (C, E and Additional file 1A). Transwell and wound healing assays showed that overexpression of ALKBH5 suppress the capability of migration and invasion in 97H and HLF (F, H and Additional file 1B, C). In contract, knockdown of ALKBH5 promoted migration and invasion in LM3 (G, I and Additional file 1B, C). GAPDH was used as internal controls in Western blotting analysis. *P < 0.05, **P < 0.01, ***P < 0.001