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Fig. 5 | Experimental Hematology & Oncology

Fig. 5

From: Dlk1 maintains adult mice long-term HSCs by activating Notch signaling to restrict mitochondrial metabolism

Fig. 5

N-acetyl-l-cysteine (NAC) recused the defective phenotypes of HSCs caused by Dlk1 knockout. A Schematic diagram of NAC incubation with bone marrow cells during in vitro culture. The SLAM HSCs (LSK, CD150+ and CD48−) were gated for following analysis. B–C Frequency in TNC and the absolute number of SLAM HSCs per legs. D Analysis of mitochondrial mass by Mitotracker green in HSCs (n = 3). E Analysis of mitochondrial membrane potential by TMRE in HSCs (n = 3). F Analysis of mitochondrial superoxide by MitoSOX Red in HSCs (n = 3). Data were expressed as mean ± SD; *p < 0.05; **p < 0.01

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Experimental Hematology & Oncology

ISSN: 2162-3619

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