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Fig. 3 | Experimental Hematology & Oncology

Fig. 3

From: Dlk1 maintains adult mice long-term HSCs by activating Notch signaling to restrict mitochondrial metabolism

Fig. 3

Dlk1 knockout in adult mice HSCs dampened long-term hematopoietic repopulation potential. A 2 × 105 donor-derived BMMCs from Dlk1 WT and KO mice (CD45.2) 6 months post poly I:C injection were transplanted with 2 × 105 rescue cells (CD45.1) into irradiated recipients. At 16 weeks posttransplant, 1 × 106 BMMCs isolated from 1st recipients were transplanted into 2nd recipients. B PB was analyzed for percent donor repopulation at the indicated number of weeks after transplants in Dlk1 WT and KO 1st and 2nd recipients (n = 6). C The absolute number of donor-derived BM cells in Dlk1 WT and KO 1st recipients were analyzed (n = 6). D PB of 1st recipients were analyzed for percent mature donor-derived lineage cells at 12-week posttransplant (n = 6). E–J Frequency in TNCs and absolute numbers of HSPCs (E and F), progenitors (G and H) and lineage cells (I and J) in Dlk1 WT and KO 1st recipient mice (n = 6). K–P Frequency in TNCs and absolute numbers of HSPCs (K and L), progenitors (M and N) and lineage cells (O and P) in Dlk1 WT and KO 2nd recipients (n = 6). Data were expressed as mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001

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