Fig. 2
Dlk1 knockout in adult mice HSCs dampened long-term hematopoietic repopulation potential. A Schematic diagram of the ELDA assay. 2 × 105, 7.5 × 104 or 2.5 × 104 donor-derived bone marrow mononuclear cell from WT or Dlk1 KO mice (CD45.2) 1 month post poly I:C injection were transplanted with 2 × 105 rescue cells (CD45.1) into irradiated recipients. B Peripheral blood (PB) of 2 × 105 group was analyzed for percent donor repopulation at the indicated number of weeks after transplants (n = 6). C The absolute number of donor-derived BM cells was analyzed in Dlk1 WT and KO 1st recipients at the end of ELDA assay (n = 6). D PB of the mice at the end of ELDA assay was analyzed for percent mature donor-derived lineage cells (n = 6). E–J Frequency in total nuclear cells (TNCs) and absolute numbers of HSPCs (E and F), progenitors (G and H) and lineage cells (I and J) in Dlk1 WT and KO 1st recipient mice (n = 6). K At 16 weeks posttransplant, 1 × 106 BMMCs isolated from 1st recipients were transplanted into 2nd recipients. CRU frequency was determined using ELDA (Extreme Limiting Dilution Analysis). L PB of 7.5 × 104 group in 2nd recipients were analyzed for percent donor repopulation at the indicated number of weeks post-transplantation (n = 6). M Absolute number of donor-derived BM cells in Dlk1 WT and KO 2nd recipients (n = 6). N–O Frequency in TNCs and absolute numbers of HSPCs in 2nd recipients were analyzed (n = 6). Data were expressed as mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001