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Fig. 1 | Experimental Hematology & Oncology

Fig. 1

From: Dlk1 maintains adult mice long-term HSCs by activating Notch signaling to restrict mitochondrial metabolism

Fig. 1

Dlk1 was highly expressed in LT-HSCs and Mx1-Cre-mediated Dlk1 knockout in mice increased number of LT-HSCs. A–C The expression of Dlk1 was analyzed in 17 mice hematopoietic cell types in a previous study from us, and was validated by flow cytometry (B) and qRT-PCR (C). D Western blot result validating that Dlk1 was almost completely knocked out after poly I:C inducement. The LSK were lysed for protein loading. E–L The changes in bone marrow mononuclear cells (E), frequency and absolute number of HSPC (F and G), cell cycle state of HSC (H), frequency and absolute number of progenitor (I and J) and frequency and absolute number of linage cells (K and L) in primary Dlk1 KO and WT mice after poly I:C inducement (n = 5). Data were expressed as mean ± SD; *p < 0.05; **p < 0.01. WT indicated wild-type mice; KO indicated knockout mice; TNC indicated total nuclear cells. n.s. indicated no significance

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