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Fig. 6 | Experimental Hematology & Oncology

Fig. 6

From: CTP synthase 2 predicts inferior survival and mediates DNA damage response via interacting with BRCA1 in chronic lymphocytic leukemia

Fig. 6

BRCA1 interacted with CTPS2 and rescued the CTPS2 knockdown phenotype in CLL cells. A The co-localization of endogenous CTPS2 and BRCA1 proteins was analyzed in MEC-1 and EHEB cells by immunofluorescence microscopy. B Co-immunoprecipitation demonstrated that CTPS2 and BRCA1 could be coprecipitated. IgG was used as the negative control. C The relative ratio of BRCA1 mRNA in CLL patients versus that in purified normal CD19 + B cells derived from healthy donors. D Correlation analysis (Pearson’s correlation) between CTPS2 and BRCA1 expression in CLL patients. E The relative expression of CTPS2 in LV-BRCA1 and LV-Control cells. F, G Validation of BRCA1 expression levels by qPCR and western blot. H, I BRCA1 overexpression rescued the shCTPS2 induced proliferation defects and apoptosis elevation. J–L BRCA1 corrected the shCTPS2-induced DNA damage. M BRCA1 overcame the downregulation of Bcl-2 and the upregulation of p-ATM as well as p-H2AX after CTPS2 knockdown. Each experiment was repeated three or more times independently. *p < 0.05, **p < 0.01, ***p < 0.001

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