Fig. 5

CTPS2 knockdown attenuated DNA repair potential in CLL. A The heatmap of differentially expressed (DE) mRNAs detected by RNA-seq in CLL cells with CTPS2 interference. B, C GO enrichment analysis and correlation network of DE molecules was performed. D The correlation analysis of CTPS2 and DDR-related genes. E Representative comet assay images of both two cell lines and quantification results. F p-H2AX immunofluorescent staining in MEC-1 and EHEB cells after CTPS2 knockdown. G CTPS2 silence upregulated phosphorylation of ATM and H2AX and reduced p-BRCA1 in CLL cells. Each experiment was repeated three or more times independently. *p < 0.05, **p < 0.01, ***p < 0.001