Fig. 3

CTPS2 knockdown restrained the survival of CLL cell lines. A–B Real-time RT-PCR analysis of CTPS2 mRNA levels in control vs shCTPS2 in MEC-1 and EHEB cells. C, D Expression of the CTPS2 was checked by western blot (WB) after transfecting. E, F Proliferation curves of MEC-1 and EHEB cells transfected with shCTPS2 or shControl. G Representative dot plots generated by flow cytometry analysis of CTPS2-knockdown groups versus negative control. H CTPS2 interference induced enhanced apoptosis through western blot analysis. I–K Representative results for the cell cycle distributions of MEC-1 and EHEB cells with CTPS2 knockdown. L The protein expression levels of cyclin B1, CDK1, p21 and CTPS2 were detected in MEC-1 and EHEB cells. Data were shown as the mean ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001