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Fig. 3 | Experimental Hematology & Oncology

Fig. 3

From: Low expression of PEBP1P2 promotes metastasis of clear cell renal cell carcinoma by post-transcriptional regulation of PEBP1 and KLF13 mRNA

Fig. 3

PEBP1P2 participates in the stable maintenance of PEBP1 mRNA. a, b PEBP1 mRNA levels were determined after transfection with shRNAs and overexpression vector by real-time PCR. c Assay model for MS2-RIP. d Real-time PCR was performed on RNA derived from MS2-RIP samples. The result of real-time PCR was normalized based on IgG control. e PEBP1 mRNA enrichment in PEBP1P2 complex was assessed by RNA pulldown assay and real-time PCR, and the mRNA enrichment was quantified by NC probe. f, g Luciferase activity of PEBP1-full-length and the PEBP1-mutation with the depletion of the binding site to PEBP1P2 was measured after transfected with indicated lentivirus. h–k Real-time PCR was performed to determine the stability of PEBP1 mRNA and GAPDH mRNA in 786-O and A-498 cells transfected with PEBP1P2-shRNA or overexpressing vector relative to 0 h after blocking new RNA transcription with α-amanitin. The data are presented as the mean ± SD, ***P < 0.001

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