Fig. 2

The expression of PEBP1P2 is inhibited by STAT4. a Following transfection with indicated shRNAs, the RNA level of PEBP1P2 was measured by real-time PCR. b Dual-luciferase reporter genes were used to evaluate PEBP1P2 promoter region activity in HEK 293 T cells transfected with the indicated shRNAs. c The activity of PEBP1P2 transcription was evaluated after transfection with indicated lentivirus via dual-luciferase reporter gene assay. d The binding affinity of STAT4 to PEBP1P2 promoter in 786-O cells was determined using a ChIP assay and real-time PCR. Real-time PCR using IgG as control was also performed. e An overview of the dCas9-gRNA-guided ChIP (left); Western blot was performed after dCas9-gRNA-guided ChIP (right). f–i The RNA and protein level of STAT4 in ccRCC and adjacent non-cancerous tissues was analyzed according to the TCGA database (f, g), clinical ccRCC samples (h) and CPTAC database (i). j Representative IHC images of STAT4 in ccRCC and adjacent tissues, respectively. k IHC score of STAT4 in ccRCC and adjacent tissues. The data are presented as the mean ± SD, **P < 0.01, ***P < 0.001