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Fig. 7 | Experimental Hematology & Oncology

Fig. 7

From: circRanGAP1/miR-27b-3p/NRAS Axis may promote the progression of hepatocellular Carcinoma

Fig. 7

circRanGAP1 promotes HCC cell progression through regulating miR-27b-3p/NRAS/ERK axis. A. Western blot assay was carried out for detecting p-ERK, ERK, and GAPDH levels in circRanGAP1-overexpressed Huh 7 cells and circRanGAP1-downregulated HCCLM3 cells and control group cells. As internal control GAPDH was used. B and C. Western blot assay, performed for evaluating p-ERK, ERK, and GAPDH levels in Huh7 and HCCLM3 cells with different treatments. D and F. Colony formation, transwell migration, and invasion assays were performed in Huh7 cells to verify the function of ERK inhibitor (SCH772984) and interfering with NRAS expression on the progression of circRanGAP1. E and G. Colony formation, transwell migration, and invasion assays were performed in HCCLM3 cells to verify the function of ERK inhibitor (SCH772984) and interfering with NRAS expression on the progression of miR-27b-3p inhibitor. The data are represented as the mean ± SD, n=3. *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant

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