Fig. 6
From: circRanGAP1/miR-27b-3p/NRAS Axis may promote the progression of hepatocellular Carcinoma
circRanGAP1 promotes HCC cell progression through regulating miR-27b-3p/NRAS axis. A and B. NRAS and CREB1 mRNA and protein levels in Huh7 cells after miR-27b-3p mimic or circRanGAP1 transfection and in HCCLM3 cells after transfection with miR-27b-3p inhibitor or circRanGAP1 shRNA. C and D. The NRAS expression in 30 HCC specimens based on circRanGAP1 and miR-27b-3p expression showed that NRAS levels in the circRanGAP1high group were greater than observed in circRanGAP1low group, whereas, NRAS levels in miR-27b-3phigh group were less than the miR-27b-3plow group. E. The binding sites of miR-27b-3p with the WT or Mut NRAS 3-UTR were predicted using StarBase v3.0. F. Luciferase activity of WT or Mut NRAS 3-UTR after transfection with miR-27b-3p mimic or inhibitor in Huh7 and HCCLM3 cells. G and H. Verification of the effects of miR-27b-3p on NRAS expression in Huh7 and HCCLM3 cells via CCK-8, Transwell migration, and invasion assays. Data are presented as the mean ± SD, n=3. *P < 0.05; **P < 0.01; ***P < 0.001; NS not significant