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Fig. 4 | Experimental Hematology & Oncology

Fig. 4

From: circRanGAP1/miR-27b-3p/NRAS Axis may promote the progression of hepatocellular Carcinoma

Fig. 4

miR-27b-3p, as a tumor suppressor, reverses circRanGAP1’s effects on HCC. A. The miR-27b-3p expression in seven HCC and normal liver cell L02. B. The relative miR-27b-3p expression in HCC and comparable normal tissues of 30 patients. C and D. Univariate and multivariate cox regression analysis of miR-27b-3p via TCGA database. E. The expression of miR-27b-3p in 30 HCC samples based on the expression of circRanGAP1, result revealed lower expression level of miR-27b-3p level in the circRanGAP1high group than that in the circRanGAP1low group. F–I. CCK-8, colony formation, transwell migration, and invasion assays were performed in HCCLM3 cells transfected with miR-NC+NC shRNA, NC shRNA+miR-27b-3p inhibitor, miR-27b-3p inhibitor+circRanGAP1 shRNA, circRanGAP1 shRNA+miR-NC, and in Huh7 cells treated with miR-NC+Mock, miR-NC+circRanGAP1, miR-27b-3p mimic+circRanGAP1, miR-27b-3p mimic +Mock. J and K. Tumor growth in Huh7 cells treated with miR-NC+Mock, miR-NC+circRanGAP1, miR-27b-3p mimic+circRanGAP1, miR-27b-3p mimic +Mock investigated by mice xenograft tumor models. The data are represented as the mean ± SD, n=3. *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant

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