Fig. 3

circRanGAP1 acted as a sponge of miR-27b-3p in HCC cells. A. Venn diagram exhibiting the overlap of circRanGAP1 target miRNAs obtained from Starbase, Tarbase, and RegRNA databases. B. AGO2 antibody was used for performing RIP experiments on extracts from Huh7 and HCCLM3 cells. C. circRanGAP1 and miR-27b-3p were evaluated by RNA FISH in Huh7 and HCCLM3 cells. Nuclei were stained with DAPI. Scale bar, 10μm. D. Putative binding site between miR-27b-3p and circRanGAP1 was predicted via StarBase v3.0. E. The luciferase activity of wildtype-circRanGAP1 or mutant-circRanGAP1 after miR-27b-3p mimic co-transfection in Huh7 cells or inhibitor in HCCLM3 cells. The data are represented as the mean ± SD, n=3. *P < 0.05; **P < 0.01; ***P < 0.001; NS not significant