Fig. 1

Generation of CAR-NK cells for adoptive transfer immunotherapy in cancer patients. A. Evolution of CAR structures from the first generation to the fourth generation according to NK cell intracellular motifs and functions. The new NK-CAR structure contains NKG2D ectodomain with CD3ζ, DAP10 or DAP12 cytoplasmic signal domain. B. The mechanisms of tumor destruction by CAR-NK cells through both CAR-dependent and CAR-independent manners. C. Different cell sources and corresponding procedures for manufacturing CAR-NK cell products