Fig. 1

PTX3 modulates the VFS-mediated LEC activation in vitro. A) Western blot analysis of MELC-2 (upper panel) and HDLEC (lower panel) treated or not with VFS mixture and rhPTX3. B-D) The effect of treatment with VFS on MELC-2 (C) and HDLEC (D) in presence or in absence of rhPTX3 was evaluated in terms of cell proliferation, cell motility in wound healing assay, sprout formation and tube formation. Scale bars: 50 μm. Data are expressed as mean ± SEM, experiments were performed in triplicate. *p < 0.05, **p < 0.01, #p < 0.001