Fig. 3

Spatiotemporal clonal evolution during the progression from MDS to AML determined by single-cell sequencing. A Experimental flow of single-cell targeted sequencing. B CCFs of shared (left), MDS-specific (middle), and AML-specific (right) mutations across all cell populations in patient P1001. C Single-cell targeted sequencing of mutations across different disease progression in patient P1001. Each column represents the sequencing results of one single cell at different stages, and the number of single cells tested in each stage is shown in parentheses. The occurrence of mutations in a same single clone is indicated by the same color as in (B). D CCFs of patient P1001 in stem cell, peripheral blood and bone marrow level. E Schematic model of clonal evolution in stem cell, peripheral blood and bone marrow level in patient P1001