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Fig. 5 | Experimental Hematology & Oncology

Fig. 5

From: Impaired mitochondria of Tregs decreases OXPHOS-derived ATP in primary immune thrombocytopenia with positive plasma pathogens detected by metagenomic sequencing

Fig. 5

Flow cytometry analysis of Th1, Th2, Th17, and Treg mitochondrial ROS and mitochondria membrane potential in pathogen( +)-ITP, pathogen(−)-ITP patients, and healthy controls. A, B Representative plots of ROS and JC-1 in pathogen( +)-ITP, pathogen(−)-ITP patient, and healthy control. C–F The MFI of ROS of Th1, Th2, Th17, and Tregs in pathogen( +)-ITP, pathogen(−)-ITP patients, and healthy controls. G–J The mitochondria membrane potential of Th1, Th2, Th17, and Tregs in pathogen( +)-ITP, pathogen(−)-ITP patients, and healthy controls. JC-1 forms aggregates emit red fluorescence while loss of membrane potential becomes monomers emit green fluorescence. The greater the accumulation of green fluorescence, the lesser the mitochondria membrane potential. Each bar graph represents mean ± SD. P values less than 0.05 were labeled in the figure. ITP immune thrombocytopenia; ROS, reactive oxygen species

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Experimental Hematology & Oncology

ISSN: 2162-3619

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