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Fig. 6 | Experimental Hematology & Oncology

Fig. 6

From: CircRIC8B regulates the lipid metabolism of chronic lymphocytic leukemia through miR199b-5p/LPL axis

Fig. 6

CircRIC8B serves as a miRNA sponge of miR-199b-5p to regulate LPL expression. A The overlapping of predicted circRIC8B target miRNAs and miRNAs targeting LPL. B miRNAs expression levels after silencing circRIC8B. C FISH assay for circRIC8B and miR-199b-5p. Scale bar, 5 μm. D Upper: Putative binding sites of miR-199b-5p concerning circRIC8B. Lower: Luciferase activity of pLG3- circRIC8B in HEK293T cells after co-transfection with miR-199b-5p. Expression levels of E miR-199b-5p and F LPL. Expression levels of G miR-199b-5p and H LPL were measured by qRT-PCR analysis after transfecting with miR-199b-5p inhibitor. I Left: Diagram of putative miR-199b-5p binding sites in the 3′-UTR of LPL. Right: Relative activities of luciferase reporters containing LPL 3′-UTR variants co-transfected with miR-199b-5p or negative control mimics in HEK293T cells. J Relative RNA levels after silencing circRIC8B. WT, wild type; Mut, Mutated; Data are shown as means ± SD (n = 3, ns, no significant, *P < 0.05, **P < 0.01, ***P < 0.001)

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