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Fig. 3 | Experimental Hematology & Oncology

Fig. 3

From: CircRIC8B regulates the lipid metabolism of chronic lymphocytic leukemia through miR199b-5p/LPL axis

Fig. 3

The characteristics of circRIC8B. A The annotated region in RIC8B gene for the formation of circRIC8B was shown. The exact sequence of the back splicing site in circRIC8B was confirmed by sequencing. B qRT–PCR for the abundance of circRIC8B and RIC8B mRNA in CLL cells treated with RNase R. Data are shown as means ± SD (n = 3, ***P < 0.001). C qRT–PCR for the abundance of circRIC8B and RIC8B mRNA in CLL cells treated with actinomycin D at the indicated time points. Data are shown as means ± SD (n = 3, **P < 0.01, ***P < 0.0001). D Nuclear and cytoplasmic fraction assay for the determination of the location of circRIC8B and RIC8B in CLL cells. U6 were applied as positive controls. E RNA-FISH for circRIC8B. circRIC8B was stained with cy3 labeled probe. Nuclei were stained with DAPI. Scale bar, 10 μm. Data are shown as means ± SD (n = 3, **P < 0.01, ***P < 0.001)

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