Fig. 1From: JOSD2 regulates PKM2 nuclear translocation and reduces acute myeloid leukemia progressiona mRNA levels of JOSD1 and JOSD2 in peripheral blood mononuclear cells (PBMCs) from primary AML patients compared with normal PBMCs (M0 = 3, M1 = 5, M2 = 5, M3 = 3, M4 = 3, M5 = 5, M6 = 3, normal n = 24). Data are presented as mean ± SD, and were analyzed by using One-way ANOVA. **P < 0.01, ***P < 0.001. b Western blot analysis of JOSD1 and JOSD2 protein expression in PBMCs from health donors and primary AML patients. &represents a band of nonspecific signals. c Cell viability of HL60, U937 and NB4 leukemia cells after JOSD1 and JOSD2 overexpression was measured by CCK-8 assay. Data are presented as mean ± SD, and were analyzed by using the 2-tailed Student t test. **P < 0.01, ***P < 0.001. d Western blot analysis of protein expression as shown in the figure after restoring JOSD2 expression in HL60, U937 and primary AML cells. e Immunopurification and mass spectrometric analysis of JOSD2-interacting proteins in JOSD2-overexpressed HL60 cells. f Co-immunoprecipitation assay showing the interaction between JOSD2 and PKM2 in JOSD2-overexpressed HL60 cells. g JOSD2 and PKM2 were co-located in the cytoplasm of HL60 cells overexpressing JOSD2. h Western blot analysis of PKM2 expression after restoring JOSD2 expression in HL60 cells. i Immunofluorescence assay shows that JOSD2 blocks PKM2 nuclear localization in HL60 cells. Scale bar, 20 μm. j The number of PKM2cells in cytoplasm or nucleus was counted. k The localization of PKM2 in HL60 cells with low JOSD2 expression and high JOSD2 expression was detected by nuclear cytoplasmic separation. Data are presented as mean ± SD, and were analyzed by using the 2-tailed Student t test. ***P < 0.001. l Western blot analysis of c-myc and cyclin D1 expression after restoring JOSD2 expression in HL60 cells. m The effects of JOSD2 on K433 acetylation and phosphorylation of S37 and Y105 of PKM2 were detected by westernblot. n JOSD2 or NC transfected HL60 cells were injected into B-NDG mice (n = 6 in NC grounp, n = 7 in JOSD2 grounp) through the tail vein. The survival was analyzed by Mantel-Cox-log-rank test. ***P < 0.001. o The spleen of leukemia-mice was weighed at day 16. Data are presented as mean ± SD, and were analyzed by using the 2-tailed Student t test. ***P < 0.001. p H&E staining of liver and spleen from mice bearing leukemia at day 16. Scale bar, 200 μmBack to article page