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Fig. 5 | Experimental Hematology & Oncology

Fig. 5

From: A sumoylation program is essential for maintaining the mitotic fidelity in proliferating mantle cell lymphoma cells

Fig. 5

Inhibition of SUMO conjugation during S phase is required for proper mitotic division in MCL cells A Jeko (left) or Z-138 (right) cells were synchronized with palbociclib (500 nM) and treated with either DMSO or TAK-981 (100 nM) and lysates were prepared at the indicated time points after washout. The corresponding phase of the cell cycle based on the DNA profiles obtained from fixed, PI stained cells from each of the time points is shown. Lysates were blotted for SUMO1, SUMO2/3, SAE1, SAE2, UBC9, and GAPDH (loading control). B Jeko cells were synchronized with Palbociclib (500 nM) for 24 h and washed from drug. Cells were either treated with DMSO or TAK-981 immediately after Palbociclib washout (t = 0) or prior to the start of G2M (17 h) (Top) Schematic showing the time of addition of TAK-981 for each experimental condition. (Bottom) DNA profiles as a function of time. C (Top) Jeko cell were synchronized with Palbociclib (500 nM, 24 h) followed by drug washout and treatment with nocodazole (50 ng/mL) either in the presence of DMSO or TAK-981 (100 nM) for 24 h. Both drugs were then washed out and cells were collected every 2 h for cell cycle analysis and for protein for SUMOylation levels. (Bottom) Jeko cell were synchronized with Palbociclib (500 nM, 24 h) followed by drug washout and treatment with nocodazole (50 ng/mL) for 24 h. Cells were then treated with either DMSO or TAK-981 for 3 h. Cells were then washed from both drugs and treated with either DMSO or TAK-981 and cells were collected every 2 h for cell cycle analysis and for protein for SUMOylation levels

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