Fig. 2

The sumoylation inhibitor TAK-981 leads to loss of sumoylation and cell death in MCL cell lines and patient samples A Normal donor resting and activated B-cells, MCL cell lines (top) and primary MCL samples (bottom) were treated with DMSO, 50, and 100 nM of TAK-981. Percent of total viable cells relative to DMSO treatment was determined. (n = 3 independent experiments for each, * p < .05). Bottom left, For each patient sample, the presence of TP53 or ATM mutation or the indicated clinical characteristic is shaded in red. Gray indicates incomplete data. B MCL cell lines were treated with DMSO, 50 nM or 100 nM of TAK-981 for 24 h. Cell lysates were blotted for total sumoylated proteins with an anti-SUMO1 (top) or anti-SUMO2/3 (bottom) antibody. C. NSG mice were engrafted with 1 × 10⁷ of either Jeko cells (left) or MCL PDX cells (right) and treated starting on day 14 (Jeko) or day 28 (PDX) post engraftment with either vehicle (n = 5) or TAK-981 7.5 mg/kg intravenously twice weekly (n = 10). Kaplan–Meier analysis shows that TAK-981 treatment yielded a statistically significant increase in survival compared to controls in both models (p < 0.001)