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Fig. 1 | Experimental Hematology & Oncology

Fig. 1

From: A sumoylation program is essential for maintaining the mitotic fidelity in proliferating mantle cell lymphoma cells

Fig. 1

Sumoylation is significantly upregulated in MCL cells and is essential for survival. A B-cells were isolated from normal peripheral blood and activated for 3 days (See Methods). Lysates were prepared and blotted for the indicated proteins. B Dot plots of the relative expression levels of UBA2 (SAE2), SAE1, and UBE2I (UBC9) within different cell subtypes from peripheral blood samples from four patients with MCL with leukemic disease. C MCL cell lines (left, n = 8) and primary MCL cells isolated from the peripheral blood of patients with leukemic disease (right, n = 5) and normal donor resting B-cells were blotted for the indicated proteins. D Survival curves of MCL patients (n = 102) stratified by the upper and lower 50th percentile of expression of SAE1, SAE2, and UBC9 using the R2 Genomic analysis and visualization platform. E Z-138 (left) UPN-1 cells (middle) or Jeko cells (right) were transduced with lentiviruses containing short hairpins targeting SAE2. After 2 days of puromycin selection, lysates were prepared and blotted for SAE1, SAE2, UBC9, SUMO1 or SUMO2. Three days after removal from selection, total viable cells were quantified. (n = 3 independent experiments for each, * p < .05)

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