Fig. 2

CML-RAE-1γ-Dex promoted NK-cell activation and proliferation. a-e Flow cytometric analysis of CD69 (a), CD137 (b), CD107a (c), perforin (d) and GzmB (e) expression in NK cells after exposure to exosomes for 6 h. f NK-cell proliferation after 72 h of culture with Dex was evaluated by flow cytometric analysis. *p < 0.05 vs. the PBS group. g, h NK cells were treated with exosomes for 6 h and then seeded in ELISPOT plates overnight. ELISPOT assays were employed to compare TNF-α and IFN-γ production by activated NK cells among the groups. i A cytotoxicity assay was used to measure the killing ability of effector NK cells prestimulated with exosomes for 6 h against BP210 (left) or BP210-T315I (right) target cells. Cytotoxic activity was detected at the effector-to-target (E:T) ratios of 12.5:1, 25:1, 50:1, and 100:1, respectively. Values are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. the mock group