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Fig. 1 | Experimental Hematology & Oncology

Fig. 1

From: Modified dendritic cell-derived exosomes activate both NK cells and T cells through the NKG2D/NKG2D-L pathway to kill CML cells with or without T315I mutation

Fig. 1

CML-RAE-1γ-Dex were isolated from DCs loaded with lysates of RAE-1γ-expressing CML cells. a Flow cytometric analysis of the expression of RAE-1γ in parental and transfected CML cells. b Flow cytometric analysis of the percentages of cells with positive expression of CD11c, CD80, CD86 and MHC class I/II before and after cytokine induction. c The morphology of Dex was visualized by TEM. Scale bar, 100 nm. d The particle size of Dex was measured by NTA. e The expression of HRS, Alix, TSG101, Cytochrome C, and RAE-1 in DCs and various types of Dex was measured by western blot analysis. f NK cells were incubated with Dex labeled with PKH26 (red). After fixation, the nuclei were stained with DAPI (blue). Finally, representative images were obtained by confocal microscopy. Red fluorescent particles represent PKH26-labeled Dex. Scale bar, 5 μm. Values are presented as the mean ± SD. *p < 0.05, ***p < 0.001

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Experimental Hematology & Oncology

ISSN: 2162-3619

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