Fig. 4

Apoptosis induced by KW-2478 is relevant to mitochondrial dysfunction. a The changes in mitochondrial membrane potential were detected by flow cytometry after CML cells were stained with JC-1. b The ratio of red fluorescence to green fluorescence was summarized after flow cytometry detection. c Representative images of JC-1 fluorescence imaging. The scale bar represents 50 μm. d Cytochrome C released from mitochondria was detected by western blot. e Western blotting was used to detect the expression level of the Bcl-2 protein family