Fig. 3

KW-2478 induced apoptosis by activating the caspase pathway in CML cells. a After K562 and K562/G01 cells were treated with KW-2478 for 48 h, flow cytometry was used to detect cell apoptosis after annexin-V-FITC/PI labelling. b The cell apoptosis rates were summarized after flow cytometry detection. c The morphology of apoptotic cells was examined after DAPI staining. The scale bar represents 10 μm. d The expression levels of cell apoptosis-related proteins, such as PARP, pro-caspase-8, cle-caspase-9 and caspase-3, were quantitatively analysed by western blots after exposure to KW-2478 for 48 h. β-Actin expression served as a loading control