Fig. 4

Summary of the reconstitution of T lymphopoiesis in vivo by transient Runx1 and Hoxa9 expression. Specific TFs combination of Runx1 and Hoxa9 were screened from several important TFs, which robustly drive the T-lineage specification. The iR9-PSCs differentiate into iHECs by mesoderm specification and HEC induction. And the OP9-DLL1 stomal cells promote T-lineage commitment with the transit expression of Runx1 and Hoxa9. PSC-derived iHPCs can be transplanted into B-NDG mice for T lymphopoiesis in the thymus, and differentiate into different functional T subsets, as the classical T-cell developmental pattern