Fig. 3

Schematic diagram of the differentiation strategies to generate T lineage subsets from PSCs. a iPSC-derived “γδ-like” CD19-CAR-T cell [82] is similar to the GNG4⁺CD8αα⁺ T(I) subset, which identified the phenotype of TCRαβ⁺TCRγδ⁻CD8α⁺CD8β^−/lowIL2RB⁻CCR7⁻CD62L(SELL)⁻ from the cell atlas of the human thymic development [31] (https://developmentcellatlas.ncl.ac.uk/datasets/HCA_thymus/fetal_thymus_Tcell_interactive_gene_expression_heatmap.html). b T-cell generation models in OP9-DLL1/PSC monolayer co-culture system. The ectopic-expressing Notch ligands on stromal cells enhanced the T-lineage commitment, the following immature T cells mature into CD8αα⁺ T cells or CD8αβ⁺ T cells under different culture conditions. c T-cell generation models in 3D co-culture system. 2-deoxyguanosine-treated thymus lobes, and MS5-DLL1/4-constructed ATO, can be used for 3D coculture system, which may provide a thymus-like microenvironment. MC, monolayer culture. d A scalable iPSC-to-iT platform under Ff condition