Fig. 2

scRNA-seq technology reveals the heterogeneity of PSCs and its derivatives and the complexity of the hematopoietic differentiation process of PSCs. a UMAP on the transcriptome of the PSCs and PSC-derived cells from Cheng’s lab (H1 ESC and H1 ESC-derived cells at day 2/4/6 during directed hematopoietic differentiation) and Guo’s lab (iPSCs and iPSC-derived EB cells at day 9/18/20 without adding any lineage-specific cytokines or conditions). b Schematic diagram of two types of PSC differentiation with or without lineage-specific cytokine combinations based on scRNA-seq datasets of Cheng’s lab and Guo’s lab. c A brief schematic overview of key differentiation steps (Q1, Q2, and Q3) from PSCs to T lymphocytes. pHEC primitive HEC, pErythro primitive erythroid cells, EMP erythromyeloid progenitor, dHEC definitive HEC, M-primed HPC myeloid-primed HPC, L-primed HPC lymphoid-primed HPC, MEP megakaryocyte-erythroid progenitor, Pro T progenitor T-cells