Fig. 1

Comparison of mutations and clones between HD and MDS groups. A Data include all single cells analyzed by single-cell RNA-seq. Data of single cells in the HPCs (H), neutrophils (n), monocytes (m), erythroblasts (E), megakaryocytes (M), B cells (B), and T cells (T) populations from individual donors are shown in columns. Detected mutations are shown in rows. Mutations are shown in red. WT is shown as blank. When mutation sites had <10 reads, we could not determine whether mutations exist and data are shown in gray as NA (not available). B The percentage of mutant cells among the total single cells examined per donor. Data are presented as the medians with interquartile ranges. C Number of identified mutations per donor. Data are presented as the mean ± S.E. D The clonal analysis of PT2.7 is shown as an example. Eleven distinct clones were detected based on combinations of four mutations and some clones were detected in multiple populations. The upper panel shows the number of cells detected in a clone. Clones are identified with the combination of mutations. The lower panel shows the combinations of mutations detected in each clone. E Number of clones per donor in the HD group and MDS group. F Clonal diversity examined using the Shannon diversity index. Mann–Whitney U test was used in Fig. 1B, E, F, and two-sided Student’s t test was used in Fig. 1C. ns, p > 0.05; *, p < 0.05; **, p < 0.01; and ***, p < 0.001. D–F Wild type (WT) clones without mutations were excluded