Fig. 2

The p53 independent role of Triptolide in p53 deficent AML cells. A Apoptotic Analysis of OCI-AML3 cells with vehicle (red lines) or p53 shRNA (blue lines) treated with control, Nutlin-3a, Triptolide, or their combination for 24 h. B Analysis of apoptotic cells in p53 deficient primary AML patients (n = 5) treated as indicated doses for 48 h. C Cells viability was detected in AML p53 mut/null cell lines treated as indicated doses for 48 h (mut: Kasumi-1; null: THP-1, HL-60). D The GO and hallmark pathway analysis was performed to reveal the enriched signaling for the 482 DEGs. E GSEA analysis of the annotations related to ER stress pathway. F Heatmap displayed hierarchical clustering of ER related genes among control,Triptolide alone or Triptolide in combination with Nutlin-3a. G, H Immune blot assessed the protein levels of MYC and ATF4 in OCI-AML3 (p53 wt) and THP-1 (p53 deficiency) cells exposed to LD Triptolide and Nutlin-3a alone or in combination. ns indicates not significant, *p < 0.05, **p < 0.01, ***p < 0.001, colored by corresponding single drug treatment in statistical tests