Fig. 1

The synergistic interaction of LD Triptolide and MDM2 inhibitor in p53 wt AML cells. AML cell lines harboring wild type p53 were treated with control, Nutlin-3a (N3), LD Triptolide (TPL) or their combination for 48 h (OCI-AML3, MV4-11) or 24 h (MOLM13). A Cell viability was determined with the CCK-8 kit. B Annexin-V/PI dual staining was used to analyze cell apoptosis. A schematic plan for the in vivo study. C Analysis of apoptotic cells in p53 wt primary AML patients (n = 19). D A schematic plan for the in vivo study. E Tumor volumes and weight of each group were separately measured. F Images of subcutaneous tumors were captured at the end of the experiment. G, H qRT-PCR analysis of the mRNA levels of p53, PUMA, P21, MDM2, XIAP and MCL-1 in OCI-AML3 cells exposed to Nutlin-3a or LD Triptolide treatment for 2, 8 and 16 h. Data were presented as mean ± S.D. ns indicates not significant, *p < 0.05, **p < 0.01, ***p < 0.001, colored by corresponding single drug treatment in statistical tests