Fig. 3

Validation of putative IRE1 substrates. A Exon-usage plots of KLF13, ATR, PRDM1, and RICTOR mRNAs, showing the number of reads in mock (red) and IRE1-treated (blue) samples. The black arrows represent the site of primers used in the 5´ region of the putative IRE1 substrates. Red arrows indicate the site of primers mapping the predicted cleavage site. Right panel of each exon-usage plot shows the abundance of KLF13, ATR, PRDM1, and RICTOR mRNA measured by qRT-PCR using the cDNAs synthesized with oligo (dT). B GAPDH and B2M were used as negative controls, and their mRNA was measured by qRT-PCR. All results are presented as the means ± SD of three experiments. (*p < 0.05, **p < 0.01, ***p < 0.001)