Fig. 1

Schematic representation of the cleavage reaction and RNA-sequencing procedure. Total RNA from H929 cells was subjected to in vitro cleavage by the cytoplasmic domain of human IRE1 or to a mock treatment. The RNA was reverse-transcribed with oligo-dT primers and then RNA-seq was performed. The lost 5´ side of the IRE1-mRNA substrates, represented in gray, is not reverse-transcribed, producing fewer reads in the mRNA cleaved by IRE1 than in mock treatment samples