Fig. 2

Distribution and frequency of TOX expression and co-expression with PD-1, Tim-3, and CD244 on Treg cells in BM and PB from MM patients. A The analytic logic of flow cytometry detection of TOX expression and co-expression with PD-1, Tim-3, and CD244 in Treg cells in PB from a patient with MM and a HI. B Comparison of the percentage of TOX + Treg cells (median: BM: 47.35 vs 10, P = 0.008, PB: 48.55 vs 28.45, P = 0.010), TOX + PD-1 + Treg cells (median: BM: 11.75 vs 0.75, P = 0.002, PB: 9.3 vs 4.21, P = 0.002), TOX + Tim-3 + Treg cells (median: BM: 2.045 vs 0, P = 0.002, PB: 1.04 vs 0.22, P = 0.004), and TOX + CD244 + Treg cells (median: BM: 0.48 vs 0, P = 0.303, PB: 0.37 vs 0.325, P = 0.838) in BM and PB from patients with MM and HIs. C Comparison of the percentage of TOX +, TOX + PD-1 +, TOX + Tim-3 +, and TOX + CD244 + Treg cells between PB and BM from 16 patients (P1–P16) with MM